Abstract Background Canine babesiosis (or piroplasmosis) is endemic in northern Portugal, but molecularly sigma kappa graphics confirmed cases of infection with small piroplasms have not been reported in the country.Three German shepherd dogs - a bitch and its 2-month old pup and an unrelated male - clinically suspected of piroplasmosis were assessed for babesial infection.Results Parasitemia with small piroplasms was detected by microscopy in two dogs.
All three dogs were positive by PCR and the Babesia microti-like small piroplasm (syn.Theileria annae) was identified by DNA sequencing.These are the first confirmed cases of babesiosis caused by the B.
microti-like piroplasm both in dogs from Portugal and in dogs suspected of clinical piroplasmosis outside of Spain.Conclusions Although the bitch and the male had visited dale duby obsidian knives neighboring Galicia (northwestern Spain), where the disease is endemic, incursion of this piroplasm into northern Portugal is evident and infection of the non-traveled pup was due to either vertical transmission or autochthonous tick infection.